Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (49): 7903-7907.doi: 10.3969/j.issn.2095-4344.2014.49.006

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Expression of sonic hedgehog in adult rat models of acute compressive spinal cord injury

Zhao Bin1, 2, Chen Bao1, 2, Zhao Hai-yan1, 2, Wang Shuan-ke1, 2   

  1. 1Department of Orthopedics, Second Hospital, Lanzhou University, Lanzhou 730030, Gansu Province, China; 2Gansu Provincial Institute of Bone and Joint Disease, Lanzhou 730030, Gansu Province, China
  • Revised:2014-08-30 Online:2014-11-30 Published:2014-11-30
  • Contact: Zhao Bin, M.D., Associate chief physician, Associate professor, Department of Orthopedics, Second Hospital, Lanzhou University, Lanzhou 730030, Gansu Province, China; Gansu Provincial Institute of Bone and Joint Disease, Lanzhou 730030, Gansu Province, China
  • About author:Zhao Bin, M.D., Associate chief physician, Associate professor, Department of Orthopedics, Second Hospital, Lanzhou University, Lanzhou 730030, Gansu Province, China; Gansu Provincial Institute of Bone and Joint Disease, Lanzhou 730030, Gansu Province, China
  • Supported by:

     the Fundamental Research Funds for the Central Universities, No.lzujbky-2011-143

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Abstract:

BACKGROUND: In the development of nervous system, the main effect of sonic hedgehog signaling pathway is to induce the partition of dorsal and ventral sides in the central nervous system. When above signaling pathway is destroyed, ventral neurons in the central nervous system will completely lose.

OBJECTIVE: To establish adult rat models of acute compressive spinal cord injury, to detect the expression of development-related sonic hedgehog after acute compressive spinal cord injury, and to explore its effects on neural regeneration.
METHODS: A total of 30 Sprague-Dawley rats at the same age were placed in a posterior compression device to establish models of acute compressive spinal cord injury. They were then randomly divided into lamina drilling group (n=5) and acute spinal cord injury group (n=25). Sonic hedgehog mRNA was detected by in situ hybridization method at the injury site at 1, 3, 5, 7, and 14 days after spinal cord.
RESULTS AND CONCLUSION: The expression levels of sonic hedgehog increased to the maximal expression levels in grey and white matters 10 mm distal to the lesion site at 7 days after injury. Sonic hedgehog expression was apparently less in ependymal cells than in grey and white matters and was restricted to 5 mm distal to the lesion site, being narrower in its distribution than its occurrence in grey and white matters. These data indicated that acute compressive spinal cord injury can induce the expression of sonic hedgehog. This expression maybe relates to adult neural cell regeneration.


中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

Key words: hedgehogs, ligands, spinal cord injuries, nerve regeneration

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